Reduction of ALL cells in the blood and spleens of mice engrafted with human ALL and treated with RAD001, vincristine, or the combination of both for 7 days. Mice engrafted with ALL-1999, -1345, or -0398 were treated with vehicle, RAD001, vincristine, or RAD001 plus vincristine for 7 days. (A) Representative flow cytometric analysis showing the percentage of human ALL cells in the PB of mice engrafted with ALL-1345. The percentage human ALL cells is shown. (B) Photographs of spleens recovered from mice engrafted with ALL-1345 after the indicated days of treatment with RAD001 or vehicle. (C) The absolute number of human ALL cells in the PB and spleens of mice treated as indicated are shown. Bars represent the mean plus or minus SD of groups of between 2 and 4 mice. *P < .05 compared with vehicle-treated animals.