RAD001 inhibits ALL cell engraftment in NOD/SCID mice. NOD/SCID mice, injected with 3 to 5 × 10⁶ cells from indicated xenografts, were treated with RAD001 starting 1 day after the injection of cells. (A) The percentage of human CD19⁺ cells (mean ± SD) in the PB at weekly bleeds of animals for each group is shown. (B) The total number of human CD19⁺ cells in the PB, BM, and spleen for each animal at death as determined by flow cytometry. The bar represents the mean of the group. (C) Infiltration of livers with human ALL. Hematoxylin and eosin staining was used. Arrows indicate ALL cells. Original magnification ×200 (details in “Histology”). Larger images are available in Figure S1.