Construction of an mKng1-targeting vector. Gene disruption was accomplished using recombineering, with details concerning the construction of the mKng1-targeting vector provided in “Methods.” Briefly, the strategy used a 9-kb genomic fragment of BAC452G1 to construct a targeting vector with which an mKng1 knockout allele was created. In this allele, exon 10 of mKng1 was replaced by a PGKNeo cassette (exon 10 contains the splicing site for HK and LK; thus, disruption of this exon should prevent transcription of HK and LK mRNA).