Characterization of transplanted human fetal mesenchymal stem cells and experimental design. (A) Immunostaining for hematopoietic markers (CD14, CD34, and CD45), markers associated with pluripotency and telomerase activity (Nanog, Oct-4, and hTERT), MSC-associated markers (CD105, CD73, CD90, and STRO-1), adhesion molecules (CD44, CD29), and matrix proteins (vimentin and laminin). Original magnification, 40×/0.75 NA oil objective. (B) Cells were transplanted intraperitoneally in oim fetuses and analyzed 1, 2, 4, 8, and 12 weeks after birth. MSC indicates mesenchymal stem cells; A, bioluminescence; B, engraftment; C, fractures; D, 3-point bending; E, cortical thickness; F, bone length; and G, growth plate analysis.