RINF expression during normal myelopoiesis. (A) Cytokine-induced (IL3 and G-CSF at 20 ng/mL, SCF at 50 ng/mL) granulocytic differentiation of myeloid CD34⁺ cells (from a healthy donor). For cell morphology (scale bar, 25 μm), cells were spread on a glass slide by cytospin, air-dried, and stained with MGG at different time points of culture (from day 2 to day 10). For each day of culture recorded, the main stage of myelopoiesis observed is indicated. At day 10, most of the cells were terminally differentiated into polynuclear neutrophil granulocytes. In our experimental conditions, a few monocytic cells were also noticed (not shown). (B) Relative mRNA expression of various genes (measured by quantitative RT-PCR) during cytokine-induced granulocytic differentiation of CD34⁺ progenitors (± SEM).