Immunophenotypic characterization of the CD8⁺ T-cell population. Flow cytometric analysis of JAK3-WT versus JAK3-AV thymic (A) or splenic (B) CD8⁺CD4⁻ T cells. (C) Flow cytometric staining for splenic T cells (CD3⁺) and B cells (CD19⁺) in JAK3-AV versus JAK3-WT mice. All analyses are gated on GFP⁺ cells and are representative of at least 5 independent experiments. Numbers indicate the percentage of cells. (D) Southern blot analysis from splenic genomic DNA of 1 JAK3-WT (lane 1) and 5 different JAK3-AV mice (lanes 2-6) using a GFP-specific probe. (E) PCR for the different variable TCRβ regions from splenocyte-derived cDNA of 1 JAK3-WT and 2 JAK3-AV animals (right panel). Each lane represents 1 PCR reaction with a different forward primer for the TCRβ variable regions (1-19 from left to right) and the same reverse primer for the constant region.