BAFF 60-mer is conserved across species. (A) Alignment of BAFF from various species, and of human TNF family members, in the region of the DE loop. Opossum:Monodelphis domestica (GenBank:XP_001375808). Platypus:Ornithorynchus anatinus (GenBank:XP_001514753). Chicken:Gallus gallus (GenBank:NM_204327). Frog:Xenopus tropicalis (EMBL:42:1929088:1912327). Fish:Danio rerio(GenBank: XM_684671). (B) Gel permeation chromatography analyses of naturally processed mouse BAFF in supernatants of 293T cells transfected with full-length mouse BAFF. Elutions were performed at pH 7.4 or pH 5, and the elution positions of molecular weight standards (in kDa) are indicated at the top of the figure. Fractions were analyzed by Western blotting using a cross-reactive antihuman BAFF antibody (Buffy-2).