Strontium-treated mice as donors or recipients in HSC transplantation settings. (A) CD45.2 BM cells from control or mice treated with Sr for 4 or 8 weeks were mixed with equal amounts of CD45.1 BM cells and were transplanted into lethally irradiated CD45.2/CD45.1 recipient mice (n = 4). Eight weeks after transplantation, the percentage of the CD45.2 population was determined into the PB of the recipients by FACS analysis. Data are mean (± SD). (B) C57BL/6 mice were treated with Sr for 4 weeks and then received transplants of 4 × 106 BM cells before lethal irradiation. Continuous Sr treatment occurred for 1, 2, 3, 4, 6, and 8 weeks after transplant, and blood cell numbers were measured in the PB. An untreated group was used as controls. The number of white blood cells (i), red blood cells (ii), and platelets (iii) are shown. Eight weeks after transplantation recipient mice were killed, and BM cells were assessed for frequency of the Lin− Sca1+ c-kit+ Flk2− cells (C) and CFU-C frequency (D). Data are mean (± SD; n = 8 per group). *** P < .001; * P < .05.
