Strontium does not affect HSC. (A) The frequency of Lin⁻ Sca1⁺ c-kit⁺ Flk2⁻ cells in the BM of untreated and Sr-treated mice was determined by FACS analysis. Values are mean (± SD; n = 4 and 8 per group for 6 and 8 weeks of treatment, respectively). (B) The cell- cycle profile of purified Lin⁻ Sca1⁺ c-kit⁺ obtained from the BM of mice treated with Sr for 8 weeks and untreated mice was defined by staining with propidium iodide and FITC and FACS analysis (representative analysis from 4 different experiments).