Mir-196b is regulated by Mll during ES cell differentiation, similar to Hoxa9 and Hoxa10. (A,B) Quantitative RT-PCR of Hoxa9 (A) and Hoxa10 (B) mRNA using SYBR green. All values are compared with WT day 0 and this value is set to 1. Experiments were performed in triplicate and the results represent average fold change plus or minus SD. (C) Quantitative RT-PCR of mir-196b expression using mir-196b TaqMan primers and probe.