HA-1 mRNA expression levels in response to 5-AZA-CdR or TSA treatment. (A) HA-1 mRNA expression levels were determined in different cell lines by qPCR after treatment with 1 μM 5-AZA-CdR for 72 hours or with 500 nM TSA for 24 hours; x-axis indicates tumor cell lines with nonmethylated or methylated HA-1 promoter and fibroblasts; y-axis, increase of the HA-1 mRNA expression levels after treatment in relation to unstimulated conditions. (B) HA-1 mRNA expression levels were determined in different cell lines with increasing doses and durations of 5-AZA-CdR treatment; x-axis indicates doses (1, 10, and 100 μM) and durations (3 and 8 days) of 5-AZA-CdR treatment; y-axis, increase of the HA-1 mRNA expression levels after treatment in relation to unstimulated conditions. Error bars reflect SEM. Values for gene expression that do not show error bars reflect data in which the SEM was too small to be depicted graphically.