Differential production of cytokines by monocytes, macrophages, and dendritic cells. Freshly isolated human PBMCs, macrophages differentiated after 5-day incubation with 10% human plasma, or dendritic cells differentiated after 5 days GM-CSF (50 ng/mL) + IL-4 (20 ng/mL), were stimulated with culture medium, LPS (100 ng/mL), Pam3Cys (10 μg/mL), or S epidermidis (10⁶ organisms/mL). IL-1β (A,B), TNF (C), or IL-1α (D) was measured in the supernantant after 24-hour incubation. Data are presented as mean plus or minus SEM of cells harvested from 6 volunteers. *P < .01 compared with the stimulation in monocytes.