Localization of basophils, eosinophils, and mast cells in the spleen, lung, and intestine after N brasiliensis infection. (A) Arrows indicate the localization of eosinophils (left panel; Siglec-F⁺IL-4/eGFP⁺B220⁻), basophils/mast cells (middle panel; IgE⁺IL-4/eGFP⁺B220⁻), and mast cells (right panel; c-Kit⁺B220⁻) in the spleen of 4get mice that had been infected with N brasiliensis 10 days before. T indicates T cell zone; B, B-cell follicle; and MZ, marginal zone. The dashed line indicates the marginal sinus. (B) Localization of eosinophils (left panel; MBP⁺ cells), basophils (middle panel; IgE⁺B220⁻ cells), and lack of mast cells (right panel; c-Kit⁺ cells) in the lung of infected 4get mice. (C) Localization of eosinophils (left panel; MBP⁺ cells), basophils/mast cells (middle panel; IgE⁺B220⁻ cells), and mast cells (right panel; c-Kit⁺ cells) in the jejunum of day-10 infected wild-type (WT), mast cell–deficient c-Kit^W-sh, and IL-4/IL-13–deficient mice. Arrows indicate goblet cell hyperplasia. Scale bars indicate 200 μm.