LMO2 is required for activation but not repression of GATA-1–regulated genes. (A) Anti-LMO2 Western blot of nuclear extracts from Bcl-X_L–expressing G1E-ER4 cells transduced with vector producing an shRNA against LMO2 or empty vector (ctr). β-Actin served as loading control. (B,C) Cells were treated with estradiol (E2) for 24 hours and mRNA levels of indicated genes were determined by real-time RT-PCR. Results were normalized to Gapdh mRNA and plotted as fold change compared with untreated cells. The data are the averages of 4 independent experiments. Error bars represent SD.