Depletion of SCL from GATA-1–repressed genes. ChIP analysis using GATA-1, SCL, GATA-2, or isotype-matched control antibodies at Kit (A), Gata2 (B), the FOG-1–independent Lyl1 gene (C), and the GATA-E-box–containing genes Etv6, Sox6, and Tram2 (D). ChIP experiments were performed in parental G1E cells and estradiol-treated G1E-ER4 cells as in Figure 1. Error bars represent SDs. *Statistical significance (P ≤ .05) based on a 2-tailed t test. Numbers indicate P values. Please note that the difference in absolute signal intensity for the SCL ChIP between panel C and the other panels is due to the use of a different batch of SCL antibodies.