GATA-1 recruits SCL to active erythroid genes. ChIP analysis using GATA-1 (A), SCL (B), and GATA-2 (C) antibodies or isotype-matched control antibodies (IgG) and primers for indicated sites. Primers for 1 kb upstream of the Hbb-b1 promoter (−1 kb) served as negative control. ChIP experiments were performed in G1E cells (−GATA-1) and G1E-ER4 cells after E2 treatment for 21 to 24 hours (+GATA-1). The data are the averages of 3 or more independent experiments. Error bars represent SDs. *Statistical significance (P ≤ .05) based on a 2-tailed t test. Numbers indicate P values.