Suppressed expression of selected classical NF-κB target genes in vitro and in vivo by sHBZ. (A) Transcriptional changes of selected classical NF-κB target genes in sHBZ-expressing Jurkat cells. After stimulating the cells with PMA plus ionomycin, the levels of TNF-α, IL-8, IFN-γ, IL2RA, CCR7, IRF1, IRF4, BCL3, ICAM-1, VCAM-1, VEGF, TAP1, and GAPDH mRNA were analyzed by increasing cycles of semiquantitative RT-PCR, represented by the ramp on the left. (B) Comparison of the HBZ gene transcripts in an ATL cell, MT-4, in HBZ-transfected Jurkat cells, and in CD4⁺ thymocytes from HBZ transgenic mice. All samples were amplified over the same number of PCR cycles as shown. (C) Transcriptional changes of selected classical NF-κB target genes in CD4⁺ thymocytes from sHBZ transgenic mice. After stimulating the cells with PMA plus ionomycin, the levels of TNF-α, IL2RA, IRF1, IRF4, VCAM-1, and GAPDH mRNA were analyzed by increasing cycles of semiquantitative RT-PCR, represented by the ramp on the left. *P < .05; **P < .01.