American Society of Hematology

Figure 6

$Figure 6. HBZ up-regulates expression of PDLIM2 gene. (A) sHBZ up-regulates PMLIM2. Total RNA was extracted from sHBZ-expressing or control Kit 225 and 293FT cells. The levels of SOCS-1, Cul2, Elongin C, PDLIM2, and GAPDH mRNA were measured by semiquantitative RT-PCR. The ramp on the left represented an increasing PCR cycle number. (B) Reducing PDLIM2 expression by siRNA recovered sHBZ-mediated suppression of p65. 293FT cells were transfected with expression vectors together with PDLIM2 siRNA or control siRNA. Protein expression was analyzed by western blotting. (C) sHBZ induced the degradation of insoluble p65. 293FT cells, untreated or treated with MG132, were transfected with mycHis-sHBZ along with FLAG-p65. After 48 hours, soluble and insoluble nuclear fractions were subjected to immunblotting. The expression levels of p65, sHBZ, Sp1, and Lamin B were detected.$

HBZ up-regulates expression of PDLIM2 gene. (A) sHBZ up-regulates PMLIM2. Total RNA was extracted from sHBZ-expressing or control Kit 225 and 293FT cells. The levels of SOCS-1, Cul2, Elongin C, PDLIM2, and GAPDH mRNA were measured by semiquantitative RT-PCR. The ramp on the left represented an increasing PCR cycle number. (B) Reducing PDLIM2 expression by siRNA recovered sHBZ-mediated suppression of p65. 293FT cells were transfected with expression vectors together with PDLIM2 siRNA or control siRNA. Protein expression was analyzed by western blotting. (C) sHBZ induced the degradation of insoluble p65. 293FT cells, untreated or treated with MG132, were transfected with mycHis-sHBZ along with FLAG-p65. After 48 hours, soluble and insoluble nuclear fractions were subjected to immunblotting. The expression levels of p65, sHBZ, Sp1, and Lamin B were detected.

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