sHBZ did not affect alternative NF-κB pathway. (A) The effect of sHBZ on p52-mediated alternative NF-κB activation. Jurkat cells were cotransfected with κB-Luc, phRL-TK, pEF-p52, and pME18Sneo-sHBZ. Luciferase levels were measured after 48 hours. (B) sHBZ did not influence p52 and p100 expression. Jurkat cells were cotransfected with vectors that express Tax and sHBZ. After 48 hours, cell lysates were then subjected to immunoblot with anti-p52, Tax, and α-tubulin.