sHBZ inhibited NF-κB and AP-1 activation but did not influence the SRF pathway. Jurkat cells were cotransfected with phRL-TK and reporter plasmid pSRF-Luc (A), AP-1-Luc (B), or κB-Luc (C), respectively, with or without 1 or 3 μg pME18Sneo-sHBZ and 1 μg pCG-Tax (A,B) or 1 μg pCMV-Tag 2-p65 (C). The total amount of DNA for transfection was equalized by adding empty vectors. After 48 hours, a dual luciferase reporter assay was preformed as described in “Methods”. All the data shown are relative values of firefly luciferase normalized to Renilla luciferase and expressed as mean of a triplicate set of experiments (± SD). *P < .05; **P < .01.