LRH-1–specific CTL TB1 of AML Pt3 efficiently recognizes LRH-1⁺ CD34⁺ AML and CML progenitor cells. (A) LRH-1–tetramer⁺ CD8⁺ T cells from Pt3 were phenotyped for their expression of CD45RA, CD27, Fas, CD127, CD28, CD45RO, and CCR7. (B) Longitudinal follow-up of LRH-1–tetramer⁺ CD8⁺ T cells in vivo from Pt3 in relation to clinical outcome. Depicted is the percentage of LRH-1–tetramer⁺ T cells in the CD8⁺ T-cell population (left y-axis) and the lymphocyte count (right y-axis) together with treatments and the occurrence of chloromas. (C) Specific lysis of CD34⁺ leukemic progenitor cells with or without preincubation with IFN-γ in the presence of LRH-1–specific CTL TB1 (■) or RP1 () was determined in flow cytometry–based cytotoxicity assays. Lysis is calculated compared with medium after 2 days of coculture at an E/T ratio of 0.5:1. Data are depicted as mean plus or minus SD of triplicate wells.