The PDZ-binding motif is essential for restoring endothelial migration in Amot knockdown in zebrafish. Zebrafish embryos (transgenic for fli:GFP visualizing endothelial cells of the developing vasculature) were injected with combinations of a morpholino against Amot and mRNA transcripts from human p80Amot or human p80 with the last 3 amino acids in the PDZ-binding motif deleted (ΔYLI). (A) Fluorescent images of zebrafish 32 hours after fertilization. Embryos were injected with morpholinos and mRNA as indicated in the figure. (B) Bar diagram showing statistics of the percentage of embryos with vascular defects. MM indicates mismatched control morpholino; MO2, morpholino targeting exon 2 of Amot; Amot, human p80Amot mRNA; and ΔYLI, human p80 mRNA with the PDZ-binding motif deleted. ***P < .001.