Adhesion of WT HSPCs on MSPCs and homing of WT BMMNCs to the BM cavity. (A) WT Lin⁻c-kit⁺ cells (2 × 10⁵) were added into each well preloaded with 10⁵ cells/well WT or Fancg^−/− MSPCs in 24-well plates. Adherent cells were counted under the phase contract microscope. Data are shown as mean (± SE) from 6 individual experiments (*P < .001 for Fancg^−/− vs WT cells). (B) CFU-Cs from the tibia of Fancg^−/− recipients after lethally irradiation and 10⁶ BMMNCs injection with or without MSPC injection. □ indicates the PBS injection; ▨, Fancg^−/− MSPC injection; and ■, the WT MSPC injection. *P < .05 for comparison of Fancg^−/− MSPCs versus PBS injection, **P < .01 for comparison of WT MSPCs versus Fancg^−/− MSPCs and PBS injection. The data show the mean (± SD) of 1 representative experiment of 3 independent experiments with similar results.