Coculture of WT HPSCs with MSPCs from WT or Fancg^−/− mice. (A) Representative photomicroscopy of cobblestone-forming areas after 4-week coculture with WT (i,iii) or Fancg^−/− MSPCs (ii,iv). Panels i and ii were taken under ×10, panels iii and iv under ×40 magnification. (B) Quantitative evaluation of the number of cobblestone-forming areas per low-power field. *P < .01 for comparison of cobblestone areas in Fancg^−/− coculture versus WT coculture. (C) After 4-week coculture of WT HSPCs with WT or Fancg^−/− MSPCs, the cells were harvested, and CFU-C assays were performed. The results shown are from 5 independent experiments. *P < .01 for comparison of WT HSPC/WT MSPC coculture with WT HSPCs-Fancg^−/− MSPC coculture. Error bars represent mean (± SE).