American Society of Hematology

Figure 5

$Figure 5. Nutlin-induced p53 blocks endoreduplication and enhances apoptosis in wild-type p53 AML cells treated with MK-0457. (A,B) OCI-AML-3 cells (A) or MOLM-13 cells (B) were treated for 48 hours with 2.5 μM Nutlin-3 and 10 nM of MK-0457, either as individual agents or in combination. Data were gated on the FL2-area versus FL2-width cytogram to exclude doublets. DMSO-treated cells served as control. Combination treatment results in reduced endoreduplication with increased percentage of annexin V–positive, apoptotic cell fraction. (C) HL-60 cells were treated for 48 hours with 2.5 μM Nutlin-3 and 10 nM MK-0457, either as individual agents or in combination. Nutlin-3 did not influence the degree of endoreduplication after MK-0457 treatment. Results are representative of 3 independent experiments.$

Nutlin-induced p53 blocks endoreduplication and enhances apoptosis in wild-type p53 AML cells treated with MK-0457. (A,B) OCI-AML-3 cells (A) or MOLM-13 cells (B) were treated for 48 hours with 2.5 μM Nutlin-3 and 10 nM of MK-0457, either as individual agents or in combination. Data were gated on the FL2-area versus FL2-width cytogram to exclude doublets. DMSO-treated cells served as control. Combination treatment results in reduced endoreduplication with increased percentage of annexin V–positive, apoptotic cell fraction. (C) HL-60 cells were treated for 48 hours with 2.5 μM Nutlin-3 and 10 nM MK-0457, either as individual agents or in combination. Nutlin-3 did not influence the degree of endoreduplication after MK-0457 treatment. Results are representative of 3 independent experiments.

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