Cells with higher DNA content are resistant to MK-0457–induced apoptosis. MOLM-13 cells were treated with 10 nM MK-0457 for 36 hours (A) or 48 hours (B). The annexin V–positive fractions in correlation with DNA content were measured by flow cytometry. Data were gated on the FL2-area versus FL2-width cytogram to exclude doublets. DMSO-treated cells served as control. Results are representative of 3 independent experiments.