Vaccination with p.DOM-WT1 DNA vaccines does not lead to destruction of hematopoietic stem cells. Groups of mice were injected with the vaccines shown on days 0 and 28 (+ electroporation), or were left unimmunized. Mice were culled between days 36 and 42 and spleen and bone marrow collected. Splenocytes were used to confirm the presence of WT1-specific T cells by ELISpot; nonresponding mice were excluded from analysis (data not shown). Bone marrow cells were assessed for the frequency of (A) colony-forming units granulocyte-macrophage (CFU-GM) and (B) burst-forming units-erythroid (BFU-E). Data are a pool of 3 of 3 experiments with group means and standard deviations shown.