Inhibition of FLT3-ITD signaling by AG1295 or PKC412 leads to induction of apoptosis, cell-cycle arrest, and inhibition of AKT and ERK phosphorylation. FDC-P1/FLT3-ITD cells were treated for 24 hours with increasing concentrations of AG1295 (A) or PKC412 (B), then stained for FACS analysis with Annexin V–FITC/PI. (C) Cell-cycle analysis was performed on FDC-P1/FLT3-ITD cells after culturing with 10 μM AG1295 for 8 or 24 hours. Data from FACS analyses are mean (± SD) from 3 individual experiments. (D) Whole-cell lysates were prepared from FDC-P1/FLT3-ITD cells incubated for 24 hours in different concentrations of AG1295 and analyzed for p-AKT and p-ERK (1 representative blot is shown; n = 3).