Characterization of the MHC II–containing compartment in activated pDCs. Twenty-four–hour flu-activated pDCs were costained for MHC II and the indicated markers of the endocytic pathway and then analyzed by 3-dimensional deconvolution microscopy. White crosses represent the zone of the captured images, which was subjected to the deconvolution process Equatorial zones of the 3-dimensional reconstruction are presented excepted for the bottom row, where a single plan containing the MTOC is shown. Scale bar represents 3 μm.