The signal strength of LMP2A is decreased in the bone marrow of TgE Notch1^lox/loxCD19^+/Cre mice. Total cells isolated from the bone marrow of each indicated genotype were stained with B220 and CD5 fluorochrome-conjugated antibodies and analyzed by flow cytometry. Live cell populations, designated by forward and side scatter properties, were gated to examine CD5 expression and a representative plot is shown for each genotype (A). (B) The average results from 4 independent experiments are shown plus or minus SE. Populations are represented as the percentage of total bone marrow that is CD5 positive (percentage of total) as well as the percentage of B cells that are CD5 positive (%B220). *P = .03, **P = .02 by t test. Notch1^lox/lox, n = 5 mice; Notch1^lox/lox CD19^+/Cre, n = 5; TgE Notch1^lox/lox, n = 7; TgE Notch1^lox/lox CD19^+/Cre, n = 9.