AKT activity is involved in BAFF-induced BCL10 and BCL3 nuclear translocation. (A) AKT activity is involved in BAFF-induced BCL10 nuclear translocation. Pfeiffer cells were treated with 20 ng/mL of BAFF for 0 to 24 hours with or without 10 μM of LY294002 (1- to 24-hour pretreatment as indicated). The nuclear lysates were subjected to immunoblotting with anti-BCL10 antibody. Immunoblotting of PCNA served as a loading control. (B) AKT activity is involved in BAFF-induced BCL3 nuclear translocation. Pfeiffer cells were treated as indicated. The nuclear lysates were subjected to immunoblotting with anti-BCL3 antibody. (C) BAFF triggers BCL10/BCL3 complex. The BCL10/BCL3 complex coexists in the nucleus. BCL3- or BCL10-coimmunprecipitated complex was isolated from the nuclear lysates prepared from Pfeiffer cells with 30-ng/mL BAFF treatments. Immunoblotting (IB) of BCL3 and BCL10 in the complex was performed.