Figure 4
![Figure 4. IL-2R/IL-2 signaling regulates hTERT promoter expression. (A) IL-2 was withdrawn from 1185 and LAF cells for 4 or 24 (overnight [O/N]) hours, followed by hTERT RNA expression by RT-PCR. (B) LAF cells were cultured in the absence of IL-2 for 4 hours, and subsequently pulsed with IL-2 for 4 and 24 hours before hTERT RT-PCR analysis. Cells grown continuously in IL-2 media served as a control (first lane). (C) 1185 cells were treated with actinomycin D (5 μg/mL) in the presence or absence of IL-2 for 0, 1, 2, and 4 hours. RNA was extracted and analyzed for hTERT expression by RT-PCR. (A-C) GAPDH was used as an amplification control.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/112/7/10.1182_blood-2008-01-134692/6/zh80160822250004.jpeg?Expires=1725186835&Signature=dNU2YYBHZ~-VUp08g6skHUXYBw8aWW40CGimR7TyUuQ-J4Ze5NLZF2bDJJWi2QMURiBw65aKNP62UJalZih8B4vG2XFnnYdcLAS2R-jrnBAe~Wx8N3iypdTN0jyNKjJgrr7ASAp2SxX7n72cmkeY0F12ac9CTkewI~~G3Uh4tPt0sH3sWzoHl~wrczBLXcwfZoyizKK4GVOy~clEYZ2uf4UZKzzddQdV9NzCKufiu~8Xl8Bq5BjLNoe9AA4U~FMavzr5ea98M2AtXPxnVwJw0vfMIQNrg7T9UGo0eD8mrtmu5CbCuJ~pvRsjYCfuNq128oXQsbDrR-jF3~xEqLyf1A__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
IL-2R/IL-2 signaling regulates hTERT promoter expression. (A) IL-2 was withdrawn from 1185 and LAF cells for 4 or 24 (overnight [O/N]) hours, followed by hTERT RNA expression by RT-PCR. (B) LAF cells were cultured in the absence of IL-2 for 4 hours, and subsequently pulsed with IL-2 for 4 and 24 hours before hTERT RT-PCR analysis. Cells grown continuously in IL-2 media served as a control (first lane). (C) 1185 cells were treated with actinomycin D (5 μg/mL) in the presence or absence of IL-2 for 0, 1, 2, and 4 hours. RNA was extracted and analyzed for hTERT expression by RT-PCR. (A-C) GAPDH was used as an amplification control.
