Addition of Pam₃Cys increases activation of the PI3K-Akt pathway in polyclonal CD8 T cells. Polyclonal CD8 T cells were stimulated in vitro with plate-bound anti-CD3 antibody alone (αCD3 only), coupled with either Pam₃Cys (αCD3 + Pam₃Cys) or plate-bound anti-CD28 (αCD3 + αCD28), or left unstimulated (naive) as a control. After 6, 12, and 18 hours of stimulation, CD8 T cells were removed from culture and total cell lysates were collected for Western blot analysis of phosphorylated (pAkt) as well as total Akt, which served as a loading control. Data shown are a representative blot of 5 independent experiments.