Recruitment of the pentameric complex and ETO2 to the Pf4 promoter in L8057 cells. (A) Schematic representation of the endogenous mouse PF4 locus. Coordinates in nucleotides are with respect to the transcriptional start site. Sequence motifs for transcription factors are marked. The ↱shows the position of the transcriptional start site. The 3 end-to-end arrows above the locus (-ve, P2, and P1) represent the location of the genomic sequences amplified by Taqman real-time PCR in the chromatin immunoprecipitation assay (ChIP) in panel B. (B) ETO2 and the pentameric complex co-occupy the Pf4 locus in L8057 cells. ChIP was performed using chromatin isolated from L8057 cells and antibodies directed against (i) GATA1, (ii) ETO2, (iii) SCL, (iv) E2A, (v) LDB1, and (vi) FLI1. Immunoprecipitated material was analyzed by Taqman real-time RT-PCR. The y-axis represents the fold enrichment normalized with respect to IgG and a control locus, the Gapdh promoter, at specific sites in the Pf4 promoter region (PF4-ve, P2, and P1).