Rearrangements of the TCR-β and immunoglobulin genes. Representative Southern blot analysis of 10 enzyme-restricted splenic tumor DNA samples. Radioactive probes specific for TCR-J1, TCR-J2, IgH and Igκ were used to demonstrate variable degrees of rearrangements in these genes. The germ-line bands are indicated by arrows. Mouse ID numbers are indicated above the blots. Details on the individual animals can be found in Table S1. Vertical line has been inserted to indicate a repositioned gel lane.