Kinetics of Foxp3⁺ regulatory T-cell induction in the spleen and tumor of EG7 or BW-Sp3 tumor-bearers. Intracellular Foxp3 staining was performed on single-cell suspensions of the spleen and tumor, measured at various time points after tumor inoculation, in parallel with the kinetic analysis of the induction of MDSCs (Figure 2A). Percentages indicate the number of CD4⁺Foxp3⁺ cells within the total CD4⁺ population. Each group consisted of at least 3 individual mice. *P < .05; ns indicates not significant.