HIV-R3A infection preferentially depleted Treg cells in DKO-hu HSC mice. Cells from spleen and mLN were harvested from each HIV- or mock-infected mouse at 1 week after infection. Mouse CD45⁻ and human CD45⁺ VLD⁻CD3⁺ cells were gated to analyze CD4/CD8, CD4/CD25, and CD4/FoxP3 from spleen (Spl; A) or mLN (B). The data shown are representative of 3 independent experiments with at least 3 DKO-hu mice in each group. Summarized data of percentage of CD4⁺ cells (C), percentage of CD3⁺CD8⁻CD25⁺ cells (D), percentage of CD3⁺CD8⁻ FoxP3⁺ cells (E), and number of FoxP3⁺ cells per organ (F) in spleen and mLN of HIV-1– versus mock-infected mice are shown. Error bars indicate standard errors. *P < .05.