Influence of PHA-739358 and IM on the phosphorylation status of Bcr-Abl kinase targets. K562 cells treated with 5 μM PHA-739358 or IM for 2 hours were analyzed by intracellular flow cytometry to assess the phosphorylation status of known Bcr-Abl kinase downstream targets: CrkL (A) and Stat5 (B). MFI indicates mean fluorescence intensity. In addition, effects of PHA-739358 or IM on K562 cells after 1 hour of treatment were analyzed measuring Bcr-Abl, Stat5, CrkL, and histone H3-(Ser10) phosphorylation levels. NT indicates not treated. Inserted frames indicate repositioned gel lanes (C).