Phenotype of neointimal cells affected by fusion proteins. 2-color immunohistologic analysis of carotid artery sections from α-TFPI–Tg or α-Hir–Tg mice (A), WT mice reconstituted with BM from α-TFPI–Tg or α-Hir–Tg (B) mice, or α-TFPI–Tg or α-Hir–Tg (C) mice reconstituted with BM from WT mice, all taken on day 28 after injury. Consecutive sections were stained with anti–α-SMA (red) and either antifusion protein, anti-CD31, anti–P-selectin, or anti–E-selectin (green). Yellow indicates colocalization. Original images taken at ×100 magnification. White lines in panel C trace out the boundaries of medial fusion protein staining; the same lines have been superimposed on the other images to indicate the boundary between medial and neointimal α-SMA staining. Representative images from experiments performed more than 3 times. (D) 3-color immunohistologic analysis of single cells isolated from intima of α-TFPI–Tg (bottom row) mice. Cells were prepared after first scraping off and discarding the luminal layer. All sections were stained with DAPI (blue), anti–α-SMA (red), and (green) either anti-VWF, anti–P-selectin, or anti–E-selectin. Yellow indicates colocalization. Original images taken at ×400 magnification and then enlarged ×5 digitally. As indicated by the white lines, images of individual cells have been cut and spliced into a single frame in Adobe Photoshop. Representative of experiments performed on at least 2 independent cohorts of animals.