Immunoscope analysis of the CDR3 region of TCR specific for CPS- and FPT-specific T cells. cDNA samples from PBMCs or in vitro–expanded T cells cultures were used for PCR reaction with Vβ specific primers as outlined in “Methods,” and results were analyzed using ABI PRISM GeneScan software. Panel A shows the representative immunoscope profile of CPS-specific CD8⁺ T cells sorted from fresh PBMCs using pMHC-peptide pentamer; panels B and C show data based on in vitro–expanded T-cell lines. Data in panel A was obtained from donor 2, while data in panels B and C was acquired from donors 1 and 2, respectively. Panel D shows representative data for FPT-specific T cells sorted from fresh PBMCs using pMHC-peptide pentamer; panels E-I show data based on in vitro–cultured T-cell lines. Data presented in panels D and E are from donor 1; data in panels F-H were from donor 2; while data in panel I was from donor 6, respectively. Panel J shows a comprehensive summary of multiple CDR3 profiles for CPS- and FPT-specific T cells. Data presented in panel J is based on the analysis carried out in 3 different HLA B*3508 + individuals.