Figure 4
![Figure 4. Roles of the PI-3 kinase and the SAPK/JNK and ERK1/2 MAPK pathways in Ang-1–induced IL-8 mRNA and protein expression. (A,B) Effects of wortmannin (WM, 50 nM), LY294002 (10 μM), SP600125 (15 μM), and PD98059 (30 μM) on IL-8 mRNA levels (representative blots in panel A and means [± SEM] in panel B) measured after 1 hour of Ang-1 exposure. Vertical lines in panel A indicate that lanes were not directly adjacent to each other in the original blots. (C) Mean (± SEM; n = 3) of IL-8 protein measured after 12 hours of Ang-1 (expressed as 100%) or Ang-1 plus U0126 (30 μM), PD98059 (30 μM), LY294002 (10 μM), or SP600125 (15 μM). *P < .05 compared with Ang-1 alone.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/111/8/10.1182_blood-2007-08-110338/6/zh80080818040004.jpeg?Expires=1722640611&Signature=4b2oiO7NjnNbxaJeDQKxN9fGPja7WuGsmUsblkOAJbYKseNxopRBjlr8xFd7GMf4gnBqIxNw09ghyT4UzMlpc~tnGresI8I3rAUzRwaYvRspj1371jBVzoNazqPfSFPkdQVD1M3fxsfn-Xqj~v8Y54L7lBX1t1lljQQzpYgiK~Jobd9wPEVQAjF1xoM8e-QH1zpkm2hPFEXVuYUkUMwhvBFOIEW1c0zgKspcuPIKxSZ5ZmzvGiOo7JDyer2PrflUYpiick9wbF4QLfvQFECF~7kwV7leVHDCUOBXljSJFJcH4-gU4mQ~m7V-Gvy8sRNgMwSRbIdBvtaxq-hAJzC0Dw__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Roles of the PI-3 kinase and the SAPK/JNK and ERK1/2 MAPK pathways in Ang-1–induced IL-8 mRNA and protein expression. (A,B) Effects of wortmannin (WM, 50 nM), LY294002 (10 μM), SP600125 (15 μM), and PD98059 (30 μM) on IL-8 mRNA levels (representative blots in panel A and means [± SEM] in panel B) measured after 1 hour of Ang-1 exposure. Vertical lines in panel A indicate that lanes were not directly adjacent to each other in the original blots. (C) Mean (± SEM; n = 3) of IL-8 protein measured after 12 hours of Ang-1 (expressed as 100%) or Ang-1 plus U0126 (30 μM), PD98059 (30 μM), LY294002 (10 μM), or SP600125 (15 μM). *P < .05 compared with Ang-1 alone.
