Expression of Notch target genes in FX^−/− mice marrow cells in vitro and in vivo. Expression of Hes1 (A) and Deltex1 (B) from 4-day CMP (WT; FX^−/−, no fucose; FX^−/−, with fucose) cultured on OP9-Dll1 was compared with OP9-control cells (set at 1) in the absence or the presence of 10 μM GSI, expressed as fold increase. (C,D) Hes1 and Deltex1 expression was analyzed from freshly isolated LSK and CMP cells from WT, FX^−/− mice maintained on standard chow (FX^−/−, no fucose) and FX^−/− mice maintained on fucose-supplement chow (FX^−/−, with fucose). Data are means plus or minus SD of 4 determinations. Student t test was performed to compare the parameters obtained with GSI to those without GSI (A,B) or values obtained from FX^−/− mice to those from WT mice (C,D).