FX^−/− mice develop a myeloproliferative disorder. (A) FACS analysis of the percentage of granulocytes (Gr-1⁺), B lymphocytes (B220⁺), and erythrocytes (TER119⁺) in the bone marrow in 3- to 4-month-old control mice (WT; n = 8), FX^−/− mice reared on fucose-supplemented chow until 12 weeks of age, and then on standard chow for at least 4 weeks (n = 8; FX^−/−, no fucose), and FX^−/− mice reared on fucose-supplemented chow until use (n = 8; FX^−/−, with fucose). (B) May-Grünwald-Giemsa–stained cytospins of marrow cells showing increased segmented neutrophils () and myeloid progenitor cells () in the bone marrow of FX^−/− mice (no fucose). (C) Differential blood counts on May-Grünwald-Giemsa–stained cytospins of marrow cells showing percentage of myeloid progenitor cells at various developmental stage and mature neutrophils (WT, n = 8; FX^−/−, no fucose, n = 9; FX^−/−, with fucose, n = 9). (D,E) FACS analysis of bone marrow myeloid progenitors (CMP: Lin⁻c-kit⁺Sca-1⁻IL7R⁻CD34⁺FcγRII^low; GMP: Lin⁻c-kit⁺Sca-1⁻IL7R⁻CD34⁺FcγRII⁺; MEP: Lin⁻c-kit⁺Sca-1⁻IL7R⁻CD34^lowFcγRII^low). (F) FACS analysis of CLP compartment (Lin⁻c-kit^lowSca-1^lowIL7R⁺). Bar graphs represent the average total number of CLP cells (± SD) from 2 tibias and 2 femurs of each mouse.