Figure 1
Figure 1. Mapping of the deletions at 10p11.2. (A) Mapping of the chromosomal breakpoints in whole chromosomes in acute-type ATLLs. An analysis of the chromosomal breakpoints was performed by spectral karyotyping (SKY), and all chromosomal breakpoints were mapped in each region of the chromosomes (x-axis), as indicated at the bottom. The y-axis shows the numbers of ATLL cases with the chromosomal breakpoints in each chromosomal region. Three regions, 10p11, 14q11, and 14q32, were frequently identified with chromosomal breakpoints. (B) Physical and transcriptional maps of the region containing the chromosomal deletion at 10p11. A FISH analysis was performed on metaphase and interphase chromosomes using 53 BAC clones mapped to the chromosome bands at 10p11-12 in the human genome map of NCBI (build 36 version 126) as probes. The bars indicate the region covering each BAC clone. Horizontal bars indicate the region with hemizygous deletions in each DNA sample from the ATLL cell lines or ATLL cells from patients, which were detected by SKY and FISH or array-CGH analyses. The inverted triangles indicate the regions of chromosomal breakpoints. Closed bars indicate the region of a homozygous deletion in a DNA sample from ATLL cells (ATL090). The hatch pattern represents the minimal heterozygous deletion at 10p11.2. TCF8 through NRP1 represent the names of the genes within the region in the human genome map of NCBI (build 36 version 126). (C) FISH validation of the RP11-188L14 probe to detect the hemizygous deletion of the chromosome10p11.2 in SO4 cell line. The RP11-188L14 probe was green (FITC) and the whole chromosome painting probe was red (TRITC). FISH with RP11-188L14 shows no signal on the abnormal chromosome 10 as indicated by the arrow (i), and a DAPI photograph corresponding to the FISH picture is shown on the right side (ii). Images were captured through the oil objective lens (100×) with a CCD camera (SenSys 0400-GI; Photometrics Ltd, Tucson, AZ). Subsequent image processing was performed with the Software IPLab version 2.4.0 (BD Biosciences Bioimaging, Rockville, MD).

Mapping of the deletions at 10p11.2. (A) Mapping of the chromosomal breakpoints in whole chromosomes in acute-type ATLLs. An analysis of the chromosomal breakpoints was performed by spectral karyotyping (SKY), and all chromosomal breakpoints were mapped in each region of the chromosomes (x-axis), as indicated at the bottom. The y-axis shows the numbers of ATLL cases with the chromosomal breakpoints in each chromosomal region. Three regions, 10p11, 14q11, and 14q32, were frequently identified with chromosomal breakpoints. (B) Physical and transcriptional maps of the region containing the chromosomal deletion at 10p11. A FISH analysis was performed on metaphase and interphase chromosomes using 53 BAC clones mapped to the chromosome bands at 10p11-12 in the human genome map of NCBI (build 36 version 126 ) as probes. The bars indicate the region covering each BAC clone. Horizontal bars indicate the region with hemizygous deletions in each DNA sample from the ATLL cell lines or ATLL cells from patients, which were detected by SKY and FISH or array-CGH analyses. The inverted triangles indicate the regions of chromosomal breakpoints. Closed bars indicate the region of a homozygous deletion in a DNA sample from ATLL cells (ATL090). The hatch pattern represents the minimal heterozygous deletion at 10p11.2. TCF8 through NRP1 represent the names of the genes within the region in the human genome map of NCBI (build 36 version 126 ). (C) FISH validation of the RP11-188L14 probe to detect the hemizygous deletion of the chromosome10p11.2 in SO4 cell line. The RP11-188L14 probe was green (FITC) and the whole chromosome painting probe was red (TRITC). FISH with RP11-188L14 shows no signal on the abnormal chromosome 10 as indicated by the arrow (i), and a DAPI photograph corresponding to the FISH picture is shown on the right side (ii). Images were captured through the oil objective lens (100×) with a CCD camera (SenSys 0400-GI; Photometrics Ltd, Tucson, AZ). Subsequent image processing was performed with the Software IPLab version 2.4.0 (BD Biosciences Bioimaging, Rockville, MD).

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