LPS or poly(I:C) treatment did not increase the 8972-Dapeak. (A) At day 7 after transplantation with syngeneic BM cells (syn), mice were treated with LPS, poly(I:C), or PBS. The average value ± SE is depicted (n = 6). No effect of LPS or poly(I:C) on the 8972-Da peak was detectable, although allogeneic marrow (allo) induced an apparent increase of the 8972-Da peak. The average intensity of the peak ± SE was 4.7 ± 0.69, 5.4 ± 1.09, 4.55 ± 0.79, and 69.3 ± 7.02 on PBS, LPS, and poly(I:C) treatment of syngeneic transplantation and untreated allogeneic transplantation, respectively (n = 6). Asterisk indicates P value. (B) LPS or poly(I:C) increased serum IL-6 concentration dramatically. In a clear contrast, concentration of IL-6 was very low in allogeneic samples similar to PBS treatment (unpaired t test). The average concentration (pg/mL) of IL-6 ± SE was 1.38 ± 0.85, 32 215.5 ± 1759.3, 2087 ± 151.3, and 1.25 ± 0.13 on PBS, LPS, and poly(I:C) treatment of syngeneic transplantation and untreated allogeneic transplantation, respectively (n = 6). The difference of PBS treatment and allogeneic transplantation is not statistically significant. Asterisk indicates P value.