TLE1 and TLE4 complex with AML1-ETO. 293T cells were transfected with HA-tagged TLE1 or TLE4 along with a FLAG empty vector (pFLAG-CMV2) or FLAG-tagged AML1-ETO (pFLAG-AML1-ETO). Total cell lysates immunoblotted with anti-HA and anti-FLAG antibodies show the expression of proteins. Lysates were immunoprecipitated with anti-FLAG antibodies, and the immunocomplexes were blotted with HA antibodies to demonstrate that both TLE1 and TLE 4 can interact with AML1-ETO.