RIP140 acts as a coactivator for NFκB. (A) Transient transfection assay of RAW264.7 macrophages cotransfected with a luciferase reporter containing −1080 bp of the TNFα promoter and expression vectors for RIP140 and RelA as indicated. (B) Same as in panel A using plasmids carrying RIP140-specific or nonspecific control shRNA constructs as indicated. (C) Transient transfection assay of RAW264.7 macrophages cotransfected with pκB-Luc (containing wild-type NFκB-binding sites) or pκB-mut-Luc (containing mutated NFκB-binding sites) together with RelA and RIP140-specific or nonspecific control shRNA constructs as indicated. Data are means plus or minus SEM (n = 9). *P < .05.