Proinflammatory gene expression is impaired in primary RIP140 knockout macrophages. (A) Pathway analysis of differential gene expression in wild-type and RIP140 knockout primary macrophages. Significantly changed pathways are shown. Percentage of genes associated with the corresponding pathway among all genes within the 4 pathways indicated. (B) Real-time PCR analysis of mRNA levels for IL-1β, IL-6, TNFα, and TNFSF4, in bone marrow–derived macrophages from wild-type (wt) or RIP140 knockout (ko) littermates. (C) Real-time PCR analysis of mRNA levels for ACAA2, CPT1B, MCAD, LCAD, ACACB, IDH3A, LPL, CD36, and GAPDH in same animals as in panel B. (D,E) Real-time PCR analysis of mRNA levels for IL-6, IL-1β, TNFα, TNFSF4, and IFNB1 in bone marrow–derived macrophages from wild-type (wt) or RIP140 knockout (ko) littermates. Cells were treated with polyI:C (10 μg/mL) for 6 hours (D) or LPS (10 ng/mL) for 2 hours (E) or left untreated as indicated. Data are means plus or minus SEM (n ≥ 3, each done in duplicate). *P < .05.