Ingenuity pathway analysis predictions. Pathways important for adipogenesis, chondrogenesis, and osteogenesis are based on gene expression data. Only pathways that are significantly enriched on both day 7 and day 14 are shown. The x-axis represents the negative log of the P value (also called the significance score) calculated from the Fisher exact test. Pathways with significance score of more than 1.3 are considered significant. Contrary to the marker analysis, statistically significant genes in all 3 lineages may overlap between cells cultured for 7 and 14 days, as well as MSCs differentiating into all 3 lineages (Figure S2). For example, in our study 138 genes are associated with the MSC differentiation process but are not necessarily lineage-specific genes.