Binding of the hDectin-1b ECD to apoptotic HEK-293 cells. HEK-293 cells were UV irradiated (100 mJ) to induce apoptosis (+UV) or were left untreated (−UV). After overnight incubation, cells were stained with (open histogram) or without (solid histogram) the hDectin-1b ECD followed by detection with an antibody that recognizes the 6xHis tag of the ECD and an appropriate FITC-conjugated secondary antibody. The percentages of positive cells are shown in the upper right corner. The data shown are representative of 3 independent experiments.